论文部分内容阅读
目的：以氧化低密度脂蛋白处理培养的兔主动脉平滑肌细胞（ＳＭＣ）致细胞增殖，观察阿魏酸钠（ＳＦ）对ＳＭＣ增殖的影响。方法：应用硫代巴比妥酸比色法测定脂质过氧化物（ＬＰＯ）水平，氚胸腺嘧啶核苷（３ＨＴｄＲ）掺入实验观察ＳＭＣ的ＤＮＡ合成。结果：ＳＦ能明显降低培养液中ＬＰＯ水平的升高，并呈剂量依赖性地减少ＳＭＣ３ＨＴｄＲ的掺入量。结论：ＳＦ能明显抑制ＳＭＣ增殖，其作用机制可能与抗脂质过氧化有关。
OBJECTIVE: To investigate the effect of sodium ferulate (SF) on the proliferation of SMCs in cultured rabbit aortic smooth muscle cells (SMC) treated with oxidized low-density lipoprotein. Methods: The level of lipid peroxidation (LPO) was determined by thiobarbituric acid colorimetry, and DNA synthesis of SMC was observed by incorporation of tritiated thymidine (3HTdR). RESULTS: SF could significantly reduce the increase of LPO in culture medium and reduce the incorporation of SMC3H-TdR in a dose-dependent manner. Conclusion: SF can significantly inhibit the proliferation of SMC, and its mechanism may be related to anti-lipid peroxidation.