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目的:建立黄芩粉末X射线衍射鉴定分析技术与黄芩苷定量分析方法。方法:采用粉末X射线衍射技术获取7个不同产地或来源的黄芩样品和1个对照品的粉末X射线衍射Fourier指纹图谱,通过对黄芩苷对照品的单一成分的衍射Fou-rier图谱获取其特征标记峰;通过HPLC技术定量检测8个样品中的黄芩苷含量;分析计算黄芩粉末X射线衍射Fourier图谱中的衍射特征峰强度与黄芩苷含量关系。结果:获得黄芩中药材的对照X射线衍射指纹图谱及其对应的50个特征标记峰,衍射峰d=3.179 5(?)的相对强度与黄芩药材中黄芩苷含量呈现良好的线性关系,线性方程为Y=1.0719X-0.193,r=0.9956。结论:粉末X射线衍射不仅可以给出反映中药材全成分的Fourier指纹图谱,并可实现对中药材中有效化学成分的定量含量检测。
OBJECTIVE: To establish a method for the identification and analysis of baicalin by X-ray powder diffraction and quantitative analysis of baicalin. Methods: The powder X-ray diffraction Fourier fingerprint of Radix Scutellariae from seven different origins and sources was obtained by powder X-ray diffraction. The characteristic of the Radix Scutellariae was determined by the diffraction Fou-rier pattern of the single component of baicalin reference substance The content of baicalin in eight samples was quantitatively determined by HPLC. The relationship between the intensity of diffraction peak and the content of baicalin in X-ray diffraction Fourier spectrum of Scutellaria baicalensis were analyzed and calculated. Results: The control X-ray diffraction fingerprinting and corresponding 50 characteristic peaks of Radix Scutellariae Radix were obtained. The relative intensity of the diffraction peak d = 3.179 5 (?) Showed a good linear relationship with the baicalin content in Radix Scutellariae. The linear equation Y = 1.0719X-0.193, r = 0.9956. Conclusion: Powder X-ray diffraction can not only give the Fourier fingerprint of Chinese herbal medicines, but also can detect the quantitative contents of effective chemical constituents in traditional Chinese medicines.