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目的:探索氟化物及再矿化物对牙本质小管的封闭效果。方法:选择8个因正畸治疗拔除的前磨牙,制作牙本质暴露模型,分为4组,每组2个牙。空白对照组:牙本质暴露模型牙不作任何处理;单纯酸蚀组:离体牙模型涂320g/L正磷酸凝胶酸蚀1min后冲洗,林格氏液中保存;酸蚀序列给药组:离体牙模型经酸蚀后涂敷100g/L氟化钠凝胶1h,冲洗,吹干后涂敷再矿化凝胶1h,冲洗后置林格氏液中保存,每天一次,连续7d;非酸蚀序列给药组:按酸蚀序列给药组方法,只是序列给药前不进行酸蚀处理,每天一次,连续7d。然后应用扫描电镜观察各组标本牙横断面和纵面牙本质表面的形貌改变;应用电子能谱仪测量各实验面氟、磷、钙元素质量分布比例的变化。结果:扫描电镜图像可以看到酸蚀序列给药组及非酸蚀序列给药组的牙本质表面有大量矿物质堆积覆盖,且酸蚀序列给药组矿物质表面层均匀、厚实。电子能谱仪检测显示,横断面边缘点氟含量比例在非酸蚀序列给药组明显增高,纵面表面检测氟、钙元素的比例在酸蚀序列给药组及非酸蚀序列给药组均显著增高,以酸蚀序列给药组更显著。结论:对牙本质敏感症的治疗,若采用酸蚀--氟化物--再矿化物涂敷的治疗程序,氟元素可以向缺损面深层渗透,在暴露的牙本质上形成表面层,或者沉积,堵塞牙本质小管,从而使牙本质小管的封堵作用更加完善。
Objective: To explore the effect of fluoride and remineralization on dentinal tubules. Methods: Eight premolar teeth extracted from orthodontic treatment were selected to make dentin exposure models. They were divided into 4 groups with 2 teeth in each group. Blank control group: dentin exposed model teeth without any treatment; simple acid etching group: Detached tooth model coated 320g / L orthophosphate gel acid etching 1min rinse, Ringer’s solution stored; acid etching sequence administration group: The model of isolated teeth was coated with 100g / L sodium fluoride gel after acid etching for 1h, rinsed, dried and coated with remineralized gel for 1h, washed and set in Ringer’s solution once a day for 7 days. In the non-acid-etching sequence administration group, the acid-etching sequence administration method was used, but the acid etching treatment was not performed before the sequence administration, once a day for 7 days. Then the morphological changes of the dentin surface and the cross section of the tooth surface were observed by scanning electron microscopy. The changes of mass proportion of fluorine, phosphorus and calcium in each experimental surface were measured by electron spectrometer. Results: SEM images showed that a large number of mineral deposits were covered on the dentin surface of the acid-etching group and the non-acid-treated group, and the mineral surface layer of the acid etching group was uniform and thick. The results of EDS showed that the proportion of fluoride in the edge of the cross section was significantly increased in the non-acid etching group, and the proportion of fluorine and calcium in the longitudinal surface was higher in the acid etching group and non-acid group Were significantly higher, with acid etching sequence administration group more significant. CONCLUSIONS: For the treatment of dentin hypersensitivity, fluoride can penetrate deeper into the defect surface, form a surface layer on the exposed dentin, or deposit if treated with an acid-fluoride-remineralization coating , Blocking the dentin tubules, so that the dentinal tubules blocking effect more perfect.