本文介绍了用高效液相色谱法检测强化牛奶中维生素A乙酸酯的方法。样品经乙醇沉淀蛋白质后用石油醚萃取,氮气吹干。液相色谱条件是以YWG—C_(18)(4.6mm×250mm)作色谱柱,甲醇为流动相,流速2ml/min,在UV325nm检测。样品整个分析周期为25～30min。该方法简便快速,重现性好,回收率95%～102%,变异系数小于3.5%。 This article describes a method for the determination of vitamin A acetate in fortified milk by high performance liquid chromatography. Samples were ethanol precipitated protein with petroleum ether extraction, dry with nitrogen. The HPLC conditions were as follows: YWG-C 18 (4.6 mm × 250 mm) as the mobile phase, methanol as mobile phase, flow rate 2 ml / min and UV325 nm detection. The entire sample analysis cycle is 25 ~ 30min. The method is simple and rapid with good reproducibility. The recoveries are 95% ~ 102% and the coefficients of variation are less than 3.5%.