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目的:探讨大杯伞菌丝体培养的最佳液体培养基及其对骨骼肌疲劳的影响。方法:采用深层培养方法,筛选出较优的碳源、氮源、无机盐,设计三因素四水平的正交试验,筛选大杯伞的最适培养基;采用脉冲式电流直接刺激蟾蜍离体腓肠肌作为疲劳模型,观察大杯伞菌丝体和任氏液对蟾蜍腓肠肌收缩能力的影响。结果:最适培养基为红糖2%、麦麸3%、磷酸二氢钾0.2%;0.8、0.3mg/ml的大杯伞菌丝体能够延长肌肉收缩下降到最大收缩幅度的90%、50%和10%的时间,与对照组相比有统计学意义,0.1mg/ml的大杯伞菌丝体能够延长肌肉收缩幅度下降到最大收缩幅度的90%的时间,与对照组相比有统计学意义(p<0.05)。结论:大杯伞菌丝体对离体骨骼肌的疲劳有延缓作用。
Objective: To investigate the best liquid culture medium of Mycelia cuneata and its effect on skeletal muscle fatigue. Methods: The optimum medium of carbon source, nitrogen source and inorganic salt was screened by deep culture method. The orthogonal experiment of three factors and four levels was designed to screen the optimum culture medium of umbrella. The pulsating current was used to directly stimulate toad in vitro Gastrocnemius muscle was used as a fatigue model to observe the effects of umbrella mycelium and Ren’s solution on the contractility of toads gastrocnemius muscle. Results: The optimal culture medium was brown sugar 2%, wheat bran 3%, potassium dihydrogen phosphate 0.2%; 0.8, 0.3mg / ml mycelium of umbrella could prolong muscle contraction to 90% % And 10% of the time, compared with the control group was statistically significant, 0.1mg / ml Mycelia umbrella can extend the muscle contraction amplitude decreased to 90% of the maximum contraction time, compared with the control group Statistical significance (p <0.05). CONCLUSION: Mycelium of M. maxima delays the fatigue of isolated skeletal muscle.