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目的:研究骨碎补总黄酮对激素性股骨头坏死兔血磷、血钙及空骨陷窝率的作用机制。方法:选取实验用新西兰大白兔30只,其中8只为空白组,另22只兔经造模成功后随机选取8只为实验组,其余8只为模型组。实验组每天给予骨碎补总黄酮溶液6m L灌胃1次,连续8周,空白组与模型组每天给予6m L 0.9%氯化钠溶液灌胃1次,连续8周。8周后检测各组新西兰大白兔血磷、血钙的变化情况,并解剖出股骨头在光镜下切片计算空骨陷窝比率,进行组间比较。结果:与空白组比较,模型组血钙明显减低(P<0.05);血磷及空骨窝比率显著升高(P<0.05);与模型组比较,实验组血钙显著升高(P<0.05),血磷及空骨陷窝比率水平均显著降低(P<0.05)。结论:骨碎补总黄酮通过改善激素性股骨头坏死对血磷、血钙的变化,改善空骨陷窝率,可能促进体外成骨细胞增殖及分化成熟,抑制破骨细胞活性、促进股骨头骨再生为骨碎补总黄酮治疗激素性股骨头坏死提供了初步理论依据。
OBJECTIVE: To study the mechanism of total flavonoids of Rhizoma Drynariae on the serum phosphorus, serum calcium and empty lacuna in rabbits with steroid-induced femoral head necrosis. Methods: Thirty New Zealand white rabbits were selected, of which 8 were blank group. The other 22 rabbits were randomly selected as the experimental group after successful modeling and the remaining 8 as the model group. The experimental group was treated with total flavonoids of Rhizoma Drynariae with 6 m L once daily for 8 weeks. The rats in the blank group and model group were orally gavaged with 6 ml 0.9% sodium chloride once daily for 8 weeks. After 8 weeks, the changes of serum phosphorus and serum calcium in New Zealand white rabbits in each group were detected. The ratio of empty bone lacuna in the femoral head under light microscope was calculated and compared between groups. Results: Compared with the blank group, the level of serum calcium was significantly decreased in the model group (P <0.05), and the ratio of serum phosphorus and empty bone was significantly increased (P <0.05). Compared with the model group, the serum calcium was significantly increased (P < 0.05). The levels of serum phosphorus and empty bone lacuna were significantly decreased (P <0.05). Conclusion: Total Flavonoids of Rhizoma Drynariae can improve osteoblast proliferation, differentiation and maturation, inhibit osteoclast activity and promote femoral head skull via improving the changes of serum phosphorus and serum calcium, Regeneration of total flavonoids for the treatment of steroid-induced osteonecrosis of the femoral head provides a preliminary theoretical basis.