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未经处理的医院污水含有多种肠道致病菌,是造成人畜感染的重要因素。为配合医院污水排放标准的制订及对医院污水的排放进行卫生学监督,进行了肠道致病菌滤膜集菌一荧光抗体染色法的研究。荧光抗体法具有敏感、特异、简单并能直接作细菌形态学观察的优点,曾应用于细菌纯培养检验,但对环境样本如土壤、水等由于致病菌数量过少及某些杂质自体荧光的
Untreated hospital sewage contains a variety of intestinal pathogens, is an important factor in human and animal infections. In line with the formulation of hospital effluent discharge standards and hygiene supervision of hospital effluent discharges, a study was carried out on the gonococcal-bacteria-based fluorescent antibody staining of intestinal pathogenic bacteria. Fluorescent antibody method is sensitive, specific, simple and can be directly observed for bacterial morphology advantages, has been used in bacterial culture tests, but for environmental samples such as soil, water, etc. due to the number of pathogenic bacteria and some impurities autofluorescence of