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目的探讨甾类激素对原代培养的大鼠附睾头部上皮细胞中Bin1b表达的影响。方法不同浓度(10~(-9),10~(-8),10~(-7) mol/L)的二氢睾酮(dihydrotestosterone,DHT)、(10~(-9),10~(-8) mol/L)雌二醇(estradiol,E2)及(10~(-8),10~(-7) mol/L)地塞米松(dexamethasone,Dex)处理原代培养的附睾头部上皮细胞0~5d,分别采用1.2%琼脂糖凝胶电泳和PCR的方法检测Bin1b mRNA的表达。结果原代培养的附睾上皮细胞中Bin1b mRNA的表达呈时间依赖性减少,培养第2天Bin1b mRNA水平下调约40%,第3天下调约70%,5d时附睾上皮细胞中几乎检测不到Bin1b mRNA的表达。不同浓度的DHT对Bin1b mRNA表达的下降有不同程度的减缓,10-9 mol/L的DHT使Bin1b mRNA表达较对照细胞增加约37%(P<0.05),10~(-8) mol/L及10~(-7) mol/L DHT处理后Bin1b mRNA表达进一步的增加。不同浓度的E_2和Dex对Bin1b mRNA表达的下降无明显调节作用。结论雄激素能上调原代培养的附睾头部上皮细胞中Bin1b的表达,而雌激素和糖皮质激素没有明显的调节作用。
Objective To investigate the effects of steroid hormones on the expression of Bin1b in epididymal epithelial cells of primary cultured rats. Methods Dihydrotestosterone (DHT), (10 ~ (-9), 10 ~ (-)) in different concentrations (10 -9, 10 -8 and 10 -7 mol / L) (8) mol / L estradiol (E2) and dexamethasone (Dex) (10 -8, 10 -7 mol / L) Cells from 0 to 5 days, respectively, using 1.2% agarose gel electrophoresis and PCR detection Bin1b mRNA expression. Results The expression of Bin1b mRNA in primary cultured epididymal epithelial cells was decreased in a time-dependent manner. Bin1b mRNA was down-regulated by about 40% on day 2 and down by about 70% on day 3. Epididymal epithelial cells showed almost no detectable expression of Bin1b mRNA expression. Different concentrations of DHT slow down the decrease of Bin1b mRNA expression, while the expression of Bin1b mRNA was increased by about 37% (P <0.05) and 10 -8 mol / L And Bin1b mRNA expression was further increased after treated with 10 -7 mol / L DHT. Different concentrations of E_2 and Dex had no significant effect on the decrease of Bin1b mRNA expression. Conclusion Androgen can up-regulate the expression of Bin1b in primary cultured epididymal cephalic epithelial cells, while estrogen and glucocorticoid have no obvious regulation.