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通过比较植物DNA提取试剂盒法、SDS法、磁珠法和质粒DNA小提试剂盒法提取玉米叶片全基因组DNA的质量,确定最适用于多重荧光SSR检测的方法。结果表明,4种方法获得全基因组DNA的ODA260/ODA280平均值分别为1.92、2.16、2.22和2.00;DNA浓度平均值分别为19.16、2 050.58、69.12、53.56 ng/μL。比较发现,SDS法和植物DNA提取试剂盒法因为提取DNA杂质多和提取成本高,均不适用于多重SSR-PCR大量样本的检测;质粒DNA小提试剂盒法和磁珠法提取的全基因组DNA都适用于多重SSR-PCR检测,分别适合人工操作和机械自动化提取。
By comparing plant DNA extraction kit method, SDS method, magnetic beads method and plasmid DNA kit method to extract the quality of maize leaf genome DNA, to determine the most suitable for multi-fluorescence SSR detection method. The results showed that the average of ODA260 / ODA280 obtained by four methods were 1.92, 2.16, 2.22 and 2.00, respectively. The average DNA concentrations were 19.16, 2050.58, 69.12 and 53.56 ng / μL, respectively. The results showed that SDS method and plant DNA extraction kit were not suitable for the detection of a large number of samples by multiple SSR-PCR because of the large amount of DNA impurities extracted and the high extraction cost. The whole genome extracted by plasmid DNA kit and magnetic beads method DNA are suitable for multiple SSR-PCR tests, respectively, for manual operation and mechanical automated extraction.