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目的研究在中国汉族人群中,子宫肌瘤的发生与雌激素受体α(ERα)基因多态性之间的关系。方法采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)来扩增ERα基因序列,用限制性内切酶对193名健康女性和92名子宫肌瘤患者的ERα基因扩增产物进行酶切,测定其基因多态性。结果 ER基因XbaI基因型及PvuⅡ基因型在子宫肌瘤组与对照组之间的分布无显著性差异(P=0.862及0.918),XbaI基因型等位基因(x、X)及PvuⅡ基因型等位基因(p、P)的分布两组比较均无显著性差异(P=0.893及0.781)。结论在中国汉族人群中,ERα基因多态性与子宫肌瘤的发生并无相关性。
Objective To investigate the relationship between the occurrence of uterine fibroids and estrogen receptor α (ERα) gene polymorphism in Chinese Han population. Methods The sequence of ERα gene was amplified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The amplification products of ERα gene in 193 healthy women and 92 patients with uterine myoma were analyzed by restriction endonucleases Digestion, determination of the gene polymorphism. Results There was no significant difference in the distribution of XbaI genotypes and PvuII genotypes between Uterine fibroid group and control group (P = 0.862 and 0.918), XbaI genotype alleles (X, X) and PvuII genotype There was no significant difference in the distribution of p (P, P) between two groups (P = 0.893 and 0.781). Conclusion There is no correlation between the ERα gene polymorphism and the occurrence of uterine fibroids in Chinese Han population.