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目的通过同源筛选的方法拼接并克隆人类多重剪接RNA结合蛋白2(RBPMS2)基因。方法采用同源筛选策略,以人RBPMS2基因作为信息探针,在GeneBank数据库中进行分析,将获得的高度同源的表达序列标签(EST)用VECTOR NTI软件拼接成重叠群。通过UCSCGenome Blat Server分析,确定RBPMS2基因的染色体定位,SMART网上分析工具进行结构域预测,Unigene数据库进行表达谱分析。结果电子克隆到人类RBPMS2新基因cDNA序列,含完整的开放阅读框(ORF)。RBPMS2基因编码的多肽由209个氨基酸组成,分子量22.5KDa,等电点8.63。SMART分析显示RBPMS2蛋白包含1个RNA识别模体(RRM)结构域。RBPMS2基因位于第15号染色体,定位在15q22.31,由7个外显子和6个内含子组成。电子表达谱分析显示RBPMS2在卵细胞、受精卵、囊胚、不同发育阶段的胚胎、膀胱、肾脏等组织中高表达。结论分析并克隆到了一个新的人类RBPMS2基因。
Objective To clone and clone the human multiple splicing RNA binding protein 2 (RBPMS2) gene by homologous screening. Methods Using homologous screening strategy, the human RBPMS2 gene was used as information probe to analyze in GeneBank database. The highly homologous expressed sequence tags (ESTs) were assembled into contigs using VECTOR NTI software. Chromosomal localization of RBPMS2 gene was determined by UCSCGenome Blat Server analysis, domain prediction was performed using the SMART online analysis tool, and expression analysis was performed by Unigene database. Results The cDNA sequence of human RBPMS2 gene was cloned electronically and contained a complete open reading frame (ORF). The polypeptide encoded by the RBPMS2 gene consists of 209 amino acids with a molecular weight of 22.5 kDa and an isoelectric point of 8.63. SMART analysis revealed that the RBPMS2 protein contains 1 RNA recognition motif (RRM) domain. The RBPMS2 gene is located on chromosome 15 and is located on 15q22.31 and consists of seven exons and six introns. Electronic expression profiling showed that RBPMS2 was overexpressed in oocytes, fertilized eggs, blastocysts, embryos in different developmental stages, bladder and kidney. Conclusion A new human RBPMS2 gene was analyzed and cloned.