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目的 :研究反射亢进型神经性膀胱逼尿肌中氮能神经分布和一氧化氮 (NO)的含量变化 ,探讨其对膀胱功能的影响。方法 :采用免疫组化法测定反射亢进型神经性膀胱患儿和膀胱功能正常小儿膀胱顶部逼尿肌中一氧化氮合酶 (NOS)阳性神经分布 ;利用硝酸还原酶法测定逼尿肌中NO的含量变化。结果 :反射亢进型神经性膀胱患儿逼尿肌中NOS阳性神经数及NO的含量明显低于正常对照组 (P <0 .0 5 )。结论 :反射亢进型神经性膀胱患儿逼尿肌中氮能神经的去神经及NO含量的减少 ,可能是造成逼尿肌反射亢进的一个因素。
Objective: To study the changes of Nerve distribution and nitric oxide (NO) in the hyperreflexic neurogenic bladder detrusor muscle and its effects on bladder function. Methods: Immunohistochemical method was used to determine the distribution of nitric oxide synthase (NOS) positive neurons in detrusor muscle of children with hyperreflexic neuropathic bladder and bladder top normal bladder. Nitric acid reductase was used to determine NO Changes in the content. Results: The number of NOS positive neurons and the content of NO in detrusor muscle of patients with hyperreflexic neuropathic bladder were significantly lower than those in normal control group (P <0.05). Conclusion: The denervation of nitric nerves and the decrease of NO content in detrusor muscle of patients with hyperreflexic neuropathic bladder may be one of the factors contributing to detrusor hyperreflexia.