目的：建立裸鼠脑内人胶质瘤模型。方法：应用自制的脑内注射细胞装置，将人的Ｕ－２５１ＭＧ细胞接种于９只裸鼠脑中。分别于接种后１０，２０，３０，４０和７７ ｄ 处死动物并行病理解剖、ＧＦＡＰ免疫组化及电镜检查。结果：我们建立的模型，肿瘤在脑内生长稳定，其特性同于人脑胶质瘤。结论：接种后２０ ｄ 左右是利用该模型进行实验的最佳时机 Objective: To establish a human brain glioma model in nude mice. METHODS: Human U-251MG cells were inoculated into the brain of 9 nude mice using a self-made intracerebral injection device. Animals were sacrificed at 10, 20, 30, 40 and 77 days after inoculation. Pathological anatomy, GFAP immunohistochemistry and electron microscopy were performed. RESULTS: In the model we established, tumors grew stably in the brain and their properties were similar to human gliomas. Conclusion: The best time to experiment with this model is about 20 days after inoculation.