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应用戊型肝炎病毒（ＨＥＶ）开放读框１（ＯＲＦ１）和开放读框２（ＯＲＦ２）区的两对寡核苷酸作为内外引物，建立了逆转录－套式聚合酶链反应（ＲＴ－ｎＰＣＲ）检测ＨＥＶＲＮＡ的方法。用本法检测２只人工感染ＨＥＶ猕猴的１１份系列血清，发现该２只猕猴分别于感染后第六天和第九天ＨＥＶＲＮＡ阳转，并持续至感染后第十七天和第二十二天。检测４１份急性散发性戊型肝炎病人血清，ＨＥＶＲＮＡ阳性率为６８．３％（２８／４１），发病后１～１０天、１１～２０天和２１～３４天的血清ＨＥＶＲＮＡ阳性率分别为７２．７％（１６／２２）、７０．０％（７／１０）和５５．５％（５／９）。提示ＨＥＶ血症持续时间较短，随病程延长而下降
RT-nPCR (reverse transcriptase-nested PCR) was established using two pairs of oligonucleotides from HEV open reading frame 1 (ORF1) and open reading frame 2 (ORF2) regions as internal and external primers. Methods of detecting HEVRNA. Using this method, two HEV macaques were tested for infectivity in 11 serogroups of HEV macaques and found that the two macaques were positive for HEVRNA on day 6 and day 9, respectively, and continued until day seventeen and twenty-two day. The positive rate of HEV RNA in 41 patients with acute sporadic encephalitis was 68.3% (28/41). The positive rates of HEV RNA in serum of 1 to 10 days, 11 to 20 days and 21 to 34 days after onset were 72 .7% (16/22), 70.0% (7/10) and 55.5% (5/9). Tip duration of HEV blood is shorter, with the duration of the decline