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为了解甲型肝炎 (甲肝 )病毒 (HAV)在中国几个城市的基因型分布 ,选择浙江杭州、江苏启东、安徽铜陵、云南昆明和上海市等的甲肝病人粪便标本或血清标本 ,以逆转录 -套式聚合酶链反应 (RT nPCR)扩增合成HAVVP1/2A交接区基因区 ,并进行直接核苷酸序列分析和差异比较。结果表明 ,从这些城市甲肝病人分离到的 17株HAV株均属基因Ⅰ型 ,为IA和IB亚型 ;所有HAV株间核苷酸差异均小于 15 % ,但约 5 0 %HAV株株间核苷酸差异大于 7 5 % ,说明中国流行或散发的HAV株可能有基因IA、IB亚型同时存在。该项研究结果将为HAV的分子流行病学追踪调查提供方法和依据。
In order to understand the genotype distribution of hepatitis A virus (HAV) in several Chinese cities, we selected fecal samples or serum samples of hepatitis A patients from Zhejiang Hangzhou, Jiangsu Qidong, Anhui Tongling, Yunnan Kunming and Shanghai to reverse transcription Nucleocapsid gene region of HAVVP1 / 2A was amplified by nested polymerase chain reaction (RTnPCR), and direct nucleotide sequence analysis and difference comparison were performed. The results showed that 17 strains of HAV isolates from these urban hepatitis A patients were of genotype Ⅰ and subtype IA and IB. The nucleotide differences among all HAV strains were less than 15%, but about 50% of HAV isolates Nucleotide differences of more than 75%, indicating that Chinese popular or distributed HAV strains may have genes IA, IB subtypes exist. The results of this study will provide methods and evidence for the molecular epidemiological investigation of HAV.