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鉴于水源11类群近年来一直处于优势地位,为简化其检测手段,本研究利用RAPD技术对该类群的8个主要致病类型进行了多态性分析,以寻找其中主要流行类型的特异性分子标记。结果如下:共筛选出10个碱基随机引物190条,其中94条可得到稳定清晰的扩增图谱,用该94条引物进行RAPD分析,发现各致病类型间遗传变异丰富;以引物S1410扩增得到了水源11-4的特异性DNA条带;以引物S1412和S1304扩增得到了水源11-14的特异性DNA条带;对引物S1304扩增得到的特异性DNA条带回收、克隆和测序,设计了1对19bp/18bp的引物,并成功地将其转化为对水源11-14特异的SCAR标记。以上结果表明,通过规模筛选来寻找小麦条锈菌生理小种的特异性DNA片段,并将其转化为稳定的SCAR标记,有可能建立起中国小麦条锈菌流行生理小种的快速分子鉴定体系。
In view of the 11 groups of water sources have been in a dominant position in recent years, in order to simplify the detection means, the study uses RAPD technology to analyze the eight major pathogenic types of this group were polymorphic, in order to find one of the major epidemic types of specific molecular markers . The results were as follows: A total of 190 random primers were screened out, of which 94 were stable and clear amplification patterns. Using the 94 primers for RAPD analysis, we found that the genetic variations among the pathogenicity types were abundant. The primers S1410 The specific DNA bands of water source 11-4 were obtained. The specific DNA bands of water sources 11-14 were amplified by primers S1412 and S1304. The specific DNA bands amplified by primer S1304 were recovered and cloned Sequencing, a pair of 19 bp / 18 bp primers was designed and successfully transformed into SCAR markers specific for water sources 11-14. The above results show that it is possible to establish a rapid molecular identification system for the physiological race of wheat stripe rust by screened for specific DNA fragments of wheat stripe rust races and to transform them into stable SCAR markers .