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目的:研究中药通心络(TXL)与治疗SAMP8小鼠早期脑内淀粉样病理变化改善的相关性。方法:取4月龄雄性SAMP8小鼠,随机分为6组:SAMP8(空白对照)组、石杉碱甲(阳性对照)组、TXL低剂(治疗)组、TXL中剂(治疗)组、TXL高剂(治疗)组、SAMR1(对照)组,每组15只。于第八周末,检测海马神经元染色质聚缩情况、皮质和海马糖原沉积情况、脑内淀粉样物质沉积情况、小鼠皮质Aβ1-42寡聚体的水平。结果:(1)HE染色结果显示SAMP8组有明显的核深染现象,通心络治疗组有改善;(2)与SAMP8对照组相比,TXL中剂组、TXL高剂组淀粉样沉积均有显著性差异(P<0.01);(3)与SAMP8组相比,TXL低剂组(P<0.05)、TXL中剂组(P<0.01)和TXL高剂组(P<0.05)PAS阳性颗粒(acid-Schiff(PAS)-positive granular structures,PGS)显著减少;(4)与SAMP8相比,TXL各治疗组Aβ42寡聚体水平均有显著性降(P<0.01);(5)TXL治疗剂量与淀粉样沉积、糖原沉积具有相关性,TXL中剂量治疗即可达到改善的效果,而Aβ42寡聚体聚集水平与TXL剂量有显著的线性负相关(r=-0.856,P<0.01)。结论:通心络治疗能够明显改善SAMP8小鼠早期脑内淀粉样病变。
Objective: To study the correlation between traditional Chinese medicine Tongxinluo (TXL) and improvement of cerebral amyloid pathology in SAMP8 mice. METHODS: Four-month-old male SAMP8 mice were randomly divided into 6 groups: SAMP8 (blank control) group, Huperzine A (positive control) group, TXL low dose (treatment) group, TXL medium (treatment) group, TXL high dose (treatment) group, SAMR1 (control) group, 15 in each group. At the end of the eighth week, the chromatin accumulation, hippocampal glycogen deposition, cerebral amyloid deposition, and cortical Aβ1-42 oligomer levels in hippocampal neurons were detected. Results: (1) The results of HE staining showed that there were obvious deep staining in SAMP8 group, and there was improvement in Tongxinluo treatment group; (2) Compared with SAMP8 control group, the amyloid deposition in TXL medium group and TXL high dose group were both improved. Significant difference (P<0.01); (3) Compared with SAMP8 group, TXL low dose group (P<0.05), TXL medium dose group (P<0.01) and TXL high dose group (P<0.05) PAS positive Acid-Schiff (PAS)-positive granular structures (PGS) decreased significantly; (4) Compared with SAMP8, the Aβ42 oligomer levels in each treatment group of TXL significantly decreased (P<0.01); (5) TXL Treatment dose was correlated with amyloid deposition and glycogen deposition. TXL medium dose therapy could achieve improved results, while Aβ42 oligomer concentration was significantly negatively correlated with TXL dose (r=-0.856, P<0.01). ). Conclusion: Tongxinluo treatment can significantly improve early cerebral amyloidosis in SAMP8 mice.