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我们采用放射免疫分析法测定62例白血病患者血清叶酸(FA)及维生素B_(12)(VB_(12))含量,以探讨其含量的变化在白血病患者病情监测中的意义。 材料和方法 一、对象:白血病患者共62例(男27,女35),经血象、骨髓象及组织化学染色等检查均符合白血病诊断。年龄19～81岁,平均47岁。其中初治急性白血病(AL)30例(M_13例,M_24例,M_36例,M_45例,M_55例,ALL7例);缓解期AL13例(M_21例,M_33例,M_44例,M_52例、ALL3例);复发期AL患者7例(M_22例,M_32例,M_42例,ALL1例);慢性粒细胞性白血病(CML)12例,另设正常对照组32例(男14,女18),年龄21～56岁,平均37岁,均为在我院体检健康者。 二、方法:空腹取静脉血2ml分离血清,采用固相非煮沸双向标记免疫分析测定法,由美国Diagnostic Procluct公司提供的FA及VB_(12)放射免疫分析试剂盒,按说明书操作。用芬兰产WIA型全自动微电脑γ计数器测定。 三、统计学处理:采用线性相关分析,t检验。
We used radioimmunoassay to determine serum folic acid (FA) and vitamin B12 (VB_(12)) levels in 62 leukemia patients to explore the significance of changes in their levels in leukemia patients. Materials and Methods First, the object: A total of 62 leukemia patients (male 27, female 35), blood, bone marrow, and histochemical staining and other tests are consistent with leukemia diagnosis. Age from 19 to 81 years old, an average of 47 years old. There were 30 cases of newly diagnosed acute leukemia (AL 13) (M 13 cases, M 24 cases, M 36 cases, M 45 cases, M 55 cases, ALL 7 cases); AL 13 cases in remission period (M 21 cases, M 33 cases, M 44 cases, M 52 cases, ALL 3 cases) The relapsed AL patients in 7 cases (M_22 cases, M_32 cases, M_42 cases, ALL1 cases); 12 cases of chronic myeloid leukemia (CML), another normal control group of 32 cases (male 14, female 18), age 21 ~ 56-year-old, an average of 37 years old, are all physically healthy in our hospital. Second, the method: fasting blood serum 2ml to separate serum, using solid-phase non-boiled two-way labeled immunoassay assay, FA and VB_ (12) radioimmunoassay kit provided by the United States Diagnostic Procluct company, according to the instructions. It was measured with a Finnish-made WIA type automatic microcomputer gamma counter. Third, statistical processing: Linear correlation analysis, t test.