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目的利用新型神经再生小室模型探讨联合应用神经生长因子(NGF)和睫状神经营养因子 (CNTF)促进周围神经电生理功能恢复的效果。方法用自行研制的梭形双通道桥接管桥接36只大鼠坐骨神经10 mm缺损,根据梭形管的两支管内注入的药物不同将动物随机分为A组(两支管内均注入几丁糖凝胶)、B组(一侧支管内注入几丁糖+NGF,另一侧支管内注入几丁糖+NGF+CNTF)和C组(一侧支管内注入几丁糖+CNTF,另一侧支管内注入几丁糖+NGF+CNTF)。于术后8,12周对再生神经的大体形态、组织病理学进行观察,并对术后12周的再生神经行皮质感觉诱发电位(CSEP)、复合肌肉动作电位(CMAP)及神经传导速度(CV)测定。结果 A组两支管内再生神经纤维组织形态学及电生理功能差异无统计学意义。B 组和C组注入NGF+CNTF侧再生纤维粗细均匀,排列整齐,明显优于对照侧,CSEP、CMAP的潜伏期短、振幅高、传导速度快,两者差异有统计学意义(P<0．05)。结论 NGF与CNTF对周围神经电生理功能的恢复具有协同作用。
Objective To explore the effect of combined application of nerve growth factor (NGF) and ciliary neurotrophic factor (CNTF) on the recovery of peripheral electrophysiological function using a novel model of neuroregeneration. Methods Thirty-six sciatic nerve defects of 36 rats were bridged with a fist-shaped dual-channel bridge tube. The animals were randomly divided into group A (two chitosan tubes were injected with chitosan Group B (chitin + NGF injected into one branch and chitosan + NGF + CNTF injected into the other branch) and Group C (chitosan + CNTF injected into one branch) Intramuscular injection of chitin + NGF + CNTF). The general morphology and histopathology of regenerative nerve were observed at 8 and 12 weeks after operation. The changes of cortical sensory evoked potential (CSEP), compound muscle action potential (CMAP) and nerve conduction velocity CV). Results There was no significant difference in histomorphology and electrophysiological function of regenerated nerve fibers between two branches of group A. The regenerated fibers in group B and group C injected NGF + CNTF had the same thickness, neatly arranged, and superior to the control. CSEP and CMAP had shorter incubation period, higher amplitude and faster conduction velocity. The difference was statistically significant (P <0. 05). Conclusion NGF and CNTF have synergistic effects on the recovery of peripheral nerve electrophysiological function.