论文部分内容阅读
目前在培养人体外周血淋巴细胞染色体的方法系采用RPMI—1640加小牛血清作培养基。此法虽好,但1640为进口培养基,价钱昂贵,且不易买到,给实验工作带来一定困难。笔者试用生理盐水,内加维生素B_(12)、叶酸、辅酶A、三磷酸腺苷等培养人体外周血淋巴细胞染色体,共作了30例获得满意结果。现将方法介绍如下,供同道参考。一、培养液制备:在无菌操作下取pH7.4磷酸盐缓冲液10ml,加入药用叶酸O.5mg。溶解后,以0.9％注射用生理盐水加至100ml,混匀,再加入维生素B_(12)500μg、辅酶A500u、三磷酸腺苷(ATP)100mg、植物血凝素(PHA)100mg,以6％碳酸氢钠液词pH至7.4,然后每培养瓶内分装5ml作培养液。
Currently in the training of human peripheral blood lymphocyte chromosome method using RPMI-1640 plus bovine serum as a medium. This method is good, but 1640 for the import medium, expensive, and not easy to buy, to the experimental work to bring some difficulties. I try saline, plus vitamin B_ (12), folic acid, coenzyme A, adenosine triphosphate and other cultured human peripheral blood lymphocyte chromosome, a total of 30 cases were satisfactory results. The method is introduced below, for fellow reference. First, the culture medium preparation: Take a pH7.4 phosphate buffer solution under sterile conditions 10ml, add medicinal folic acid O. 5mg. After dissolving, the solution was added to 100 ml 0.9% physiological saline for injection, and then mixed with 500 μg of vitamin B 12, 100 mg of coenzyme A 500u, 100 mg of adenosine triphosphate (ATP) and 100 mg of phytohemagglutinin (PHA) The liquid word pH to 7.4, and then each flask bottled 5ml for culture medium.