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目的评估STRtyper-10F/G、CODIS系统联合应用在突变三联体和二联体亲子鉴定中的鉴定能力。方法104例亲子鉴定样本,采用CODIS检测,其中3例三联体发现突变的案例以及101例二联体中73例认定和28例排除案例,分别再使用STRtyper-10F/G检测;统计认定和排除情况、比较单独使用和联合使用在排除关系案例中的表现和法医学参数(H、DP、PE、PIC、TPI)。结果 3例存在突变基因的三联体案例加做STRtyper-10F/G未发现更多的矛盾基因座,PI值均大于10 000,可作出认定结论;73例二联体认定案例中13例PI不足10 000,加做STRtyper-10F/G系统后PI>10 000,可确认认定;28例排除案例最高排除率CODIS为50.00%、STRtyper-10F/G为64.29%;两个体系均具有较高的杂合度(H≥0.7)和信息量(PIC>0.7),联合应用CEP为0.999 999 999 505 3。结论 STRtyper-10F/G和CODIS系统联合应用可满足突变三联体和二联体亲子鉴定的需要。
Objective To evaluate the ability of the STRtyper-10F / G and CODIS systems to be used in the identification of mutant triads and diaries. Methods A total of 104 cases of paternity test samples were detected by CODIS. Among them, three cases of triplet mutation and 101 cases of duplex and 28 cases of exclusion were identified, and STRtyper-10F / G was used again. Statistical identification and exclusion The situation compares the performance and forensic parameters (H, DP, PE, PIC, TPI) in the exclusion case alone and in combination. Results Three cases of triplet with mutant gene plus STRtyper-10F / G did not find more contradictory loci with PI values greater than 10 000, which could be used to make the conclusion. In the 73 cases of duplex identified, 13 cases were deficient in PI 10 000, and PI> 10 000 after adding STRtyper-10F / G system, confirming the affirmation; the highest exclusion rate of 28 cases was CODIS of 50.00% and STRtyper-10F / G of 64.29%; both systems had higher Heterozygosity (H≥0.7) and information amount (PIC> 0.7), the combined application CEP was 0.999 999 999 505 3. Conclusion The combination of STRtyper-10F / G and CODIS system can meet the needs of paternity test of mutation triplets and diaries.