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应用骨髓染色体G显带技术,在450例慢性髓性白血病(CML)检出3例隐匿型Ph易位,报道如下:1 细胞遗传学分析方法 均取患者的骨髓血,细胞数按终浓度(2～3)×10~9/ml加入含20%小牛血清或20%自体血浆的McCoy’s培养液中,在37C温箱中进行24h培养,终止培养前2h按终浓度0.08～0.1μg/ml加入秋水仙素,常规低渗、固定后,进行制片、老化、G显带,方法详见报道。每例至少分析20个中期分裂相。异常核型按人类染色体国际命名体制ISCN标准定义。对每个异常克隆拍照、放大、剪排,制备3～5个核型图。2 病例及临床资料 3例皆为男性,按张之南主编的有关标准进行诊断、分期和疗效评定。 例1,42岁,1993年7月因左肋部疼痛、低热、乏力3周,在我院门诊查白细胞(WBC)80×10~9/L,肝
Three cases of occult Ph-translocation were detected in 450 cases of chronic myelogenous leukemia (CML) using bone marrow G-banding technique. The report is as follows: 1 Cytogenetic analysis of patients with bone marrow blood cells, the number of cells by the final concentration ( 2 ~ 3) × 10 ~ 9 / ml was added to McCoy’s medium containing 20% fetal bovine serum or 20% autologous plasma and incubated in a 37C incubator for 24 hours. The final concentration was 0.08 ~ 0.1μg / ml Add colchicine, conventional hypotonic, fixed, the filming, aging, G-banding, the method described in detail. Each case analyzed at least 20 metaphase. Abnormal karyotype according to the International Human Naming System ISCN standard definition. For each abnormal clone photographs, enlarge, cut the row, prepare 3 to 5 karyotype map. 2 cases and clinical data of 3 cases are all men, according to Zhang Zhinan editor of the relevant standards for diagnosis, staging and efficacy evaluation. Cases of 1,42 years old, July 1993 due to pain in the left rib cage, fever, fatigue for 3 weeks in our hospital outpatient examination white blood cells (WBC) 80 × 10 ~ 9 / L, liver