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利用 2 2个来源于小麦 (TriticumaestivumL .)和栽培大麦 (HordeumvulgareL .)的STS_PCR标记 ,研究了 32份新疆布顿大麦 (HordeumbogdaniiWilensky)的遗传多样性。在这 2 2个STS_PCR标记中 ,仅有 3个标记的扩增产物经HinfⅠ、HhaⅠ、HaeⅢ和RsaⅠ 4种限制性内切酶消化后没有产生多态性DNA片段 ,而 19个标记 (占 86 .4 % )和 4 6种标记 /酶组合 (占 5 2 .3% )能够揭示材料间的多态性。在 32份布顿大麦材料的 88种STS_PCR标记 /酶组合中 ,总共得到 315条DNA片段 ,平均每个标记 /酶组合能得到 3.6条DNA片段。在这 315条DNA片段中 ,共有 12 3条片段(占 39.0 % )具有多态性 ,每一个多态性标记 /酶组合能获得 1～ 6条多态性DNA片段。STS_PCR标记揭示的 32份布顿大麦的遗传距离变化范围为 0 .0 78～ 0 .35 2 ,平均为 0 .198。根据STS_PCR标记的遗传距离矩阵 ,采用不完全加权算术平均数法 (UPGMA)构建了 32份布顿大麦群体间的遗传关系树状图 ,结果表明STS_PCR标记能将 32份材料完全区分开来。同时 ,来源于同一地方的不同居群没有明显地聚类在一起 ,表明新疆布顿大麦的遗传多样性与其地理分布相关不紧密。
The genetic diversity of 32 Hordeumbogdanii Wilensky cultivars was studied using 22 STS_PCR markers derived from wheat (Triticum aestivum L.) and cultivated barley (Hordeum vulgare L.). Of the 22 STS_PCR markers, only 3 of the amplified products were digested with Hinf Ⅰ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ 4 restriction endonucleases and no polymorphic DNA fragments were produced. Nineteen markers (86 .4%) and 46 markers / enzyme combinations (52.3%) could reveal the polymorphism between materials. A total of 315 DNA fragments were obtained from the 88 STS PCR markers / enzyme combinations of 32 burley barley materials, with an average of 3.6 DNA fragments per tag / enzyme combination. Of the 315 DNA fragments, 12 3 fragments (39.0%) were polymorphic, and 1 to 6 polymorphic DNA fragments were obtained for each polymorphism marker / enzyme combination. The genetic distance of 32 bouton barley revealed by STS_PCR marker ranged from 0.078 to 0.352 with an average of 0.198. According to the genetic distance matrix of STS_PCR markers, the genetic relationship tree of 32 Boutun barley populations was constructed by incomplete weighted arithmetic mean (UPGMA). The results showed that STS_PCR markers could completely distinguish 32 materials. At the same time, different populations from the same place were not clustered together obviously, indicating that the genetic diversity of Burdon barley in Xinjiang is not closely related to its geographical distribution.