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采用细胞杂交瘤技术获得一株血吸虫成虫抗原的单克隆抗体SM 38-3。其靶抗原为成虫表膜,免疫球蛋白型为1gG 2a,能识别血吸虫分子量为25k D的表膜蛋白抗原表位。该单克隆抗体纯化后标记过氧化物酶,用于Dot-ELISA测定慢性血吸虫病人血清115份,阳性反应103份,阳性率为89.6％;测定急性病人血清89份,阳性反应85份,阳性率为95.5％;测定141份正常人血清,阳性反应2份,假阳性率为1.4％。与血吸虫肠相关抗原的检测比较,结果阳性率与抗原滴度均无明显差别。
A monoclonal antibody against adult schistosome schistosome SM 38-3 was obtained by using the cell hybridoma technique. Its target antigen is the adult membrane, immunoglobulin type 1gG 2a, can identify Schistosoma japonicum molecular weight 25k D epitopes of membrane protein. The monoclonal antibody was purified and labeled with peroxidase. Dot-ELISA was used to detect 115 serum samples from patients with chronic schistosomiasis. The positive rate was 89.6%. 89 serum samples were obtained from acute patients and 85 positive samples were obtained. The positive rate Was 95.5%. 141 normal human serum samples were determined, two were positive, the false positive rate was 1.4%. Compared with the detection of schistosoma gut associated antigen, the positive rate of the results and the antigen titers were not significantly different.