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通过SPA-ELISA、免疫电镜(ISEM)和种植观察,证明南方菜豆花叶病毒(SBMV)主要存在于大豆种皮中,并能引起侵染;检疫检验时,可以通过对种皮的检验来代替对整粒种子的检验,从而达到降低检验成本,提高检验快速的目的。
SPA-ELISA, immunoelectron microscopy (ISEM) and planting observation showed that S. bean mosaic virus (SBMV) was mainly present in soybean seed coat and could cause infection. In quarantine inspection, it could be replaced by test on seed coat The whole seed test, so as to reduce the cost of inspection and improve the rapid test purposes.