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Objective:To investigate the cytotoxic effect of ethanol extract of the stem bark of asam kandis[Garcinia cowa Roxb.(G.cowa)]on T47 D breast cancer cell line.Methods:The cytotoxicity of ethanol extract was carried out against human breast cancer cell line(T47D) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay.The extract was added at various concentrations(0.1.1,10 and 100 μg/mL).The level of cytotoxicity was determined by calculating the level of IC_(50),that was based on the percentage of the cell death after 24 h treatment with the extract.Cell morphological changes were observed by using inverted microscope.Results:The 3-(4.5-dimelhylthiazol-2-yl)-2.5-diphenyltelrazolium bromide assay showed that ethanol extract of G.cowa exhibited significant cytotoxic effect on T47 D with IC_(50) value of(5.10+1.68) μg/mL.Morphological alteration of the cell lines after exposure to ethanol extract of G.cowa was observed under phase contrast microscope in a dosc-dependent manner.ConclusionsThe results suggest the possible use of ethanol extract of asam kandis for preparing herbal medicine for cancer-related ailments.
Objective: To investigate the cytotoxic effect of ethanol extract of the stem bark of asam kandis [Garcinia cowa Roxb. (G.cowa)] on T47 D breast cancer cell line. Methods: The cytotoxicity of ethanol extract was carried out against human breast cancer Cell line (T47D) by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide colorimetric assay. The extract was added at various concentrations (0.1.1, 10 and 100 μg / mL) level of cytotoxicity was determined by calculating the level of IC 50 (that was based on the percentage of the cell death after 24 h treatment with the extract. Cell morphological changes were observed by using inverted microscope. Results: The 3- (4.5 -dimelhylthiazol-2-yl) -2.5-diphenyltelrazolium bromide assay showed that ethanol extract of G.cowa exhibited significant cytotoxic effect on T47 D with IC 50 value of (5.10 + 1.68) μg / mL. Morphological alteration of the cell lines after exposure to ethanol extract of G.cowa was observed under phase contrast microscope in a dosc -dependent manner.ConclusionsThe results suggest the possible use of ethanol extract of asam kandis for preparing herbal medicine for cancer-related ailments.