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为揭示植物源病毒抑制剂VFB的抗病毒机理,本研究以普通烟和烟草花叶病毒(TMV)为材料,测定了VFB进行预防和治疗处理后烟草中与抗病性相关的部分生理生化指标变化。结果表明:VFB可明显抑制病毒侵染后导致的细胞膜通透性增大;SOD、CAT、β-1,3-葡聚糖酶和几丁质酶等细胞防御酶活性增强,且预防处理的效果优于治疗处理;与抗病性呈正相关的脯氨酸和总游离氨基酸的含量有显著的提高。表明VFB可诱导植物产生抗病性,增强烟草对TMV侵染的抵抗能力。
In order to reveal the antiviral mechanism of plant-borne virus inhibitor VFB, this study measured the physiological and biochemical parameters related to disease resistance in tobacco treated and treated with VFB by using normal tobacco and tobacco mosaic virus (TMV) Variety. The results showed that: VFB significantly inhibited cell membrane permeability caused by virus infection; SOD, CAT, β-1, 3-glucanase and chitinase and other cell defense enzyme activity increased, and prophylactic treatment The effect is better than the treatment. The contents of proline and total free amino acids positively correlated with disease resistance were significantly increased. This indicated that VFB can induce plants to develop disease resistance and enhance the resistance of tobacco to TMV infection.