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目的分析沙眼衣原体主要外膜蛋白抗原的二级结构及B、T细胞表位。方法采用计算机在线预测网站PSIPRED V3.3对沙眼衣原体主要外膜蛋白抗原蛋白的二级结构进行预测和分析;采用BCEpred和ABCpred网络软件对沙眼衣原体主要外膜蛋白B细胞表位进行预测和分析;采用SYFPEITHI在线软件对沙眼衣原体主要外膜蛋白的MHC-Ⅰ类HLA-A 0201限制性T细胞表位进行预测和分析。结果沙眼衣原体主要外膜蛋白抗原蛋白的二级结构中无规则卷曲结构占32.49%、α螺旋结构占41.62%、β-折叠结构占19.80%。沙眼衣原体主要外膜蛋白抗原的B细胞表位氨基酸序列位点分别为41~50、92~101、51~60、306~315、260~269和160~169,MHC-Ⅰ类HLA-A 0201限制性T细胞表位氨基酸序列位点分别为201~209、379~387、3~11、126~134和177~185。结论沙眼衣原体主要外膜蛋白抗原的二级结构中无规则卷曲结构、α螺旋结构所占比例高,有6个B细胞表位和5个T细胞表位。
Objective To analyze the secondary structure and B and T cell epitopes of the major outer membrane antigen of Chlamydia trachomatis. Methods The secondary structure of the major outer membrane protein antigen of Chlamydia trachomatis was predicted and analyzed by computer online prediction platform PSIPRED V3.3. BCEpred and ABCpred software were used to predict and analyze the major epitopes of outer membrane protein B of Chlamydia trachomatis. The HLA-A 0201 restricted T cell epitopes of the MHC-I major outer membrane protein of C. trachomatis were predicted and analyzed using SYFPEITHI online software. Results In the secondary structure of Chlamydia trachomatis major outer membrane antigen protein, the random coil structure accounted for 32.49%, the α-helix structure accounted for 41.62% and the β-sheet structure accounted for 19.80%. The amino acid sequences of B cell epitopes of the major outer membrane protein antigens of Chlamydia trachomatis were 41-50, 92-101, 51-60, 306-315, 260-269 and 160-169, respectively. The MHC class I HLA-A 0201 Restricted T cell epitope amino acid sequence sites were 201 ~ 209,379 ~ 387,3 ~ 11,126 ~ 134 and 177 ~ 185. Conclusions The primary outer membrane protein antigen of Chlamydia trachomatis has no secondary curly structure in the secondary structure. The α-helix structure occupies a high proportion and has 6 B-cell epitopes and 5 T-cell epitopes.