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【目的】研究启膈散乙酸乙酯部位对食管癌Eca109细胞生长的影响及其作用机制。【方法】采用四甲基偶氮唑盐(MTT)法检测启膈散乙酸乙酯部位对食管癌Eca109细胞的生长抑制作用,用流式细胞仪检测细胞凋亡情况,Western blot法检测STAT3、Bcl-2、Caspase9蛋白的表达量。【结果】在10~100μg/m L范围内,不同浓度启膈散乙酸乙酯部位能有效抑制Eca109细胞增殖(P<0.05)。用1.47、33.26、76.52μg/m L的启膈散乙酸乙酯部位作用Eca109细胞48 h,细胞凋亡率显著增加,与对照组比较,差异均有统计学意义(P<0.01);Western blot检测结果显示:随着药物浓度的增加,STAT3和Bcl-2的表达量降低,Caspase9表达量增加。【结论】启膈散乙酸乙酯部位能显著抑制食管癌Eca109细胞增殖,并诱导细胞凋亡,其作用机制与抑制STAT3信号通路有关。
【Objective】 To investigate the effect of Qifang Acetate Eca109 on the growth of esophageal carcinoma Eca109 cells and its mechanism. 【Methods】 The growth inhibition of esophageal cancer Eca109 cells was detected by MTT assay. Cell apoptosis was detected by flow cytometry. The expression of STAT3, Bcl-2, Caspase9 protein expression. 【Results】 Eca109 cells were inhibited effectively by different doses of Qigezhuang ethyl acetate in the range of 10 ~ 100μg / mL. (P <0.05). The apoptosis rate of Eca109 cells treated with 1.47,33.26,76.52μg / m L of Eca109 cells for 48 hours increased significantly compared with the control group (P <0.01). Western blot The results showed that with the increase of drug concentration, the expression of STAT3 and Bcl-2 decreased and the expression of Caspase9 increased. 【CONCLUSION】 Qiqi powder ethyl acetate can significantly inhibit the proliferation and induce the apoptosis of esophageal cancer Eca109 cells. Its mechanism is related to the inhibition of STAT3 signaling pathway.