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RNA干扰(RNAinterference,RNAi)是由双链RNA(double-strandedRNA,dsRNA)介导的,序列特异的基因沉默现象,是体内抵御外来感染的一种重要保护机制。小干扰RNA(smallinterferingRNA,siRNA)是RNAi过程中的效应分子。siRNA相关技术可下调在卵巢肿瘤发生及发展过程中起关键作用的癌基因、抑癌基因、凋亡相关基因、生长因子及其受体、酶和蛋白等基因的表达,从而达到基因治疗的目的。综述siRNA的作用机制,特点及其在卵巢肿瘤治疗研究中的应用。
RNA interference (RNAi) is a double-stranded RNA (dsRNA) -mediated, sequence-specific gene silencing that is an important protective mechanism against foreign infection in vivo. Small interfering RNA (siRNA) is an effector molecule in RNAi. siRNA-related technology can down-regulate the expression of oncogenes, tumor suppressor genes, apoptosis-related genes, growth factors and their receptors, enzymes and proteins that play key roles in the development and progression of ovarian tumors so as to achieve the purpose of gene therapy . The mechanism of action of siRNA, its characteristics and its application in the treatment of ovarian cancer.