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本研究对轻度子宫内膜异位症患者（３０例）及正常对照组（１５例）在腹腔镜直视下收集腹腔液，正常人精液按１：１比例稀释，以去透明带地鼠卵穿透试验（ＨＯＰ）、精子尾部低渗肿胀试验（ＨＯＳ）及精子头部ＤＮＡ荧光染色有效精子计数（ＥＳＣ）为指标，探讨轻度子宫内膜异位症（ＥＭ）患者的腹腔液对精子受精能力、精子膜及ＤＮＡ 双螺旋结构的影响。结果显示，ＥＭ组精子穿卵率和受精卵外精子附着数明显低于正常对照组（Ｐ＜０．０１），ＥＭ组的 ＨＯＳ明显低于正常对照组（Ｐ＜０． ０５），而 ＥＭ组的 ＥＳＣ虽有所下降，但与正常对照组相比无显著性差异。结果提示：轻度ＥＭ腹腔液对精子受精能力及精子膜结构的完整性有影响。
In this study, patients with mild endometriosis (30 cases) and normal control group (15 cases) were collected peritoneal fluid under laparoscopic direct vision, normal sperm diluted 1: 1 ratio, to the zona pellucida (HOP), hypothalamus test (HOS), and effective sperm count (ESC) of fluorescent DNA in sperm head were used as indexes to investigate the effect of peritoneal fluid on patients with mild endometriosis (EM) Sperm fertilizing ability, sperm membrane and DNA double helix structure. The results showed that the rate of sperm penetration and the number of sperm oocyte attachment in EM group were significantly lower than those in normal control group (P <0.01). The HOS in EM group was significantly lower than that in normal control group (P <0.05) Although the ESC of the group decreased, there was no significant difference compared with the normal control group. The results suggest that: mild EM peritoneal fluid on sperm fertilization and sperm membrane structure integrity.