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目的 探究茶多酚 (TP)对铅 (Pb)致细胞脂质过氧化损伤的拮抗作用。方法 Wistar雄性大鼠 6 0只随机分为 6组 ,分别给予 0、5 0、10 0、30 0mg/kg不同浓度TP灌胃 ,第 6、8、10天分别给予乙酸铅 4 0mg/kg腹腔注射 ,对照组给予蒸馏水灌胃 ,腹腔注射生理盐水。第 11日眶静脉采血 ,继续TP灌胃 1周后处死动物 ,取股动 -静脉混合血、肝、脑组织实验。结果 (1)单纯染铅组大鼠体重较非染铅组明显降低 ,差异有显著性 (P <0 .0 5 ) ,而同时予TP 10 0、30 0mg/kg灌胃可明显拮抗铅对大鼠生长抑制 ,末次染铅 2 4h、1周的大鼠体重与TP相关系数为r=0 .973(P <0 .0 1)和r =0 .9979(P<0 .0 1) ,呈正相关。 (2 )末次染铅 2 4h、1周的染铅大鼠较非染铅大鼠血、肝、脑丙二醛 (MDA)含量明显升高 ,差异有显著性 (P <0 .0 5 ) ;超氧化物歧化酶 (SOD)活力抑制 ,差异有显著性 (P <0 .0 5 ) ,TP10 0、30 0mg/kg灌胃可拮抗铅的上述作用 ;同时染铅大鼠血、肝、脑谷胱甘肽 (GSH)水平、谷胱甘肽 S 转移酶 (GST)活力亦发生明显改变 ,而TP 10 0、30 0mg/kg灌胃可拮抗铅的作用。结论 TP可明显拮抗铅致大鼠血、肝、脑脂质过氧化损伤而具有防治、拮抗铅中毒的重要功能。
Objective To investigate the antagonistic effect of tea polyphenols (TP) on lipid peroxidation induced by lead (Pb) in mice. Methods Sixty male Wistar rats were randomly divided into 6 groups and given different doses of TP at 0, 50, 100, 30, 0 mg / kg respectively. On the 6th, 8th and 10th days, 40 mg / kg lead Injection, control group given distilled water, intraperitoneal injection of saline. On the 11th day, orbital venous blood was collected and animals were sacrificed one week after TP instillation. The femoral artery - vein mixed blood, liver and brain tissue were used for experiment. Results (1) Compared with non-lead group, body weight of lead-exposed rats was significantly lower than that of non-lead group (P <0.05), while administration of TP at 10,30,30 mg / kg significantly antagonized lead The growth inhibition of rats, the correlation coefficient of body weight and TP for the 4 h and 24 h after the last lead exposure were r = 0.973 (P <0.01) and r = 0.9979 (P <0.01) Was positively correlated. (2) The content of malondialdehyde (MDA) in the blood, liver and brain of the lead-exposed rats 24 h and 1 week after the last lead exposure was significantly higher than that of the non-lead lead rats, the difference was significant (P <0.05) ; The activity of superoxide dismutase (SOD) was inhibited, the difference was significant (P <0.05), TP10 0,30 0mg / kg intragastric administration can antagonize the above effects of lead; Glutathione (GSH) levels and glutathione S-transferase (GST) activity also changed significantly, while TP 10 0 and 30 mg / kg were antagonized by lead. Conclusion TP can obviously antagonize the lipid peroxidation injury of blood, liver and brain in lead-induced rats and has the important function of preventing and controlling lead poisoning.