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制备了基于溶菌酶适体竞争机理的信号减弱型电化学传感器,在玻碳电极上修饰羧基化的多壁碳纳米管,将带有氨基的互补适体DNA连接到多壁碳纳米管上。由于道诺霉素崁入电极上的互补DNA,而产生电化学信号,当有目标物时,适体与结合力更高的溶菌酶结合,原本的互补链解链,导致插入双链的道诺霉素脱落,电化学信号减弱。以微分脉冲伏安法测定溶菌酶,响应的范围为1～500 nmol/L,相关系数0.999,检测下限为0.5 nmol/L(S/N=3)。
A signal attenuated electrochemical sensor based on the competitive mechanism of lysozyme aptamer was prepared. The carboxylated multiwalled carbon nanotubes were modified on the glassy carbon electrode, and the DNA with complementary aptamers with amino groups was connected to the multiwalled carbon nanotubes. Because daunorubicin inserts into the complementary DNA on the electrode, an electrochemical signal is generated. When the target is present, the aptamer binds to the higher binding lysozyme and the original complementary strand melts, resulting in the insertion of double-stranded DNA Loss of doxorubicin, electrochemical signal attenuation. Lysozyme was determined by differential pulse voltammetry. The response range was 1 ~ 500 nmol / L, the correlation coefficient was 0.999, and the detection limit was 0.5 nmol / L (S / N = 3).