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目的建立大鼠皮肤损伤核磁共振(nuclear magnetic resonance,NMR)检测方法,探讨其在法医学中应用的意义。方法雄性SD大鼠36只,随机分为皮肤切创和挫伤组,分别在大鼠背部制作皮肤切创和挫伤,各组分别在损伤后0h、4h、8h取皮肤组织及对侧无损伤部位皮肤,检测NMR一维1H谱,观察受创皮肤的分子代谢产物随损伤时间的变化规律。结果挫伤组、切创组背部损伤处皮肤的1H-NMR图谱在损伤后4h和8h分别检测到较多NMR波峰,波峰的高度和总面积也发生了改变,与对照部位皮肤相比,存在显著性差异(P<0.05)。结论核磁共振技术检测组织代谢产物有望用于损伤经过时间的法医学检验。
Objective To establish a method for the detection of skin lesions in rats by using nuclear magnetic resonance (NMR) and to explore the significance of its application in forensic medicine. Methods Thirty-six male Sprague-Dawley rats were randomly divided into skin incision and contusion group. Skin incision and contusion were made on the back of rats, respectively. The skin tissues and contralateral non-injury sites were harvested at 0h, 4h and 8h after injury Skin, 1H NMR one-dimensional NMR spectroscopy was observed to observe the variation of the molecular metabolites of the affected skin with the injury time. Results In the contusion group and the incision group, the 1H NMR spectra of the skin at the back injury detected more NMR peaks at 4h and 8h after injury, respectively, and the peak height and total area also changed. Compared with the skin at the control site, there were significant Sex differences (P <0.05). Conclusion The detection of tissue metabolites by nuclear magnetic resonance (NMR) is expected to be used for forensic examination of the time course of injury.