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目的:观察模拟航渡及大强度运动对雄性大鼠神经-内分泌-免疫网络的影响及三七总皂苷的调控作用。方法:将6周龄的SD雄性大鼠随机分为静息对照组(QC组)、训练对照组(TC组)和三七总皂苷组(PNS组),每组10只。QC组不接受任何刺激,在生理盐水灌胃1周后即进行取样。TC组和PNS组大鼠经过10 d递增负荷跑台训练,分别生理盐水和PNS灌胃1周后,进行模拟航渡及高强度运动,结束即刻取血,使用放免法测定大鼠血清皮质酮(Cort)、促肾上腺皮质激素(ACTH)、β-内啡肽(β-EP)、神经肽Y(NPY)、白介素1β(IL-1β)和白介素6(IL-6);采用免疫组织化学方法检测各组大鼠垂体ACTH,NPY的表达水平,并进行相关分析;电镜下观察垂体、肾上腺超微结构。结果:训练对照组大鼠与静息对照组相比,血清Cort,β-EP水平显著升高,同时,垂体ACTH,NPY表达水平显著降低。灌服PNS后与TC组相比,血清Cort,ACTH及NPY水平下降;同时,免疫组化结果表明垂体ACTH,NPY表达水平也明显增高。在此基础上观察到应激后大鼠腺垂体及肾上腺皮质的超微结构改变,功能受损,灌服PNS后对细胞具有保护作用。结论:负性心理应激及大强度的运动训练使大鼠神经-内分泌-免疫网络功能紊乱,PNS对神经-内分泌-免疫网络具有调控作用。
Objective: To observe the effect of simulated flight course and intensive exercise on the neuroendocrine-immune network in male rats and the regulatory effect of Panax notoginseng saponins. Methods: 6-week-old SD male rats were randomly divided into resting control group (QC group), training control group (TC group) and Panax notoginseng saponins group (PNS group), with 10 rats in each group. The QC group did not receive any stimulation, and the samples were taken after 1 week of saline instillation. Rats in TC group and PNS group were subjected to treadmill training on increasing load for 10 d. Rats in each group were given normal saline and PNS for 1 week. After simulated flight and high-intensity exercise, blood samples were taken out immediately. Serum corticosterone Cort, ACTH, β-EP, NPY, IL-1β and IL-6 were detected by immunohistochemistry Methods The expression of ACTH and NPY in the pituitary of rats in each group were detected and analyzed. The ultrastructure of pituitary and adrenal gland were observed under electron microscope. Results: Compared with the resting control group, the serum Cort and β-EP levels in the training group were significantly increased, meanwhile, the expression of ACTH and NPY in the pituitary decreased significantly. Serum levels of Cort, ACTH and NPY decreased after PNS administration compared with TC group. Meanwhile, immunohistochemical results showed that the expression of ACTH and NPY in pituitary was also significantly increased. On this basis, the ultrastructural changes of adenohypophysis and adrenal cortex were observed in rats after stress, impaired function and protective effect on cells after PNS administration. Conclusion: Negative psychological stress and high intensity exercise training can make neuroendocrine-immune network dysfunction. PNS plays a regulatory role in neuroendocrine-immune network.