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目的利用高效液相色谱法测定接骨丸中臣药马钱子所含主要成分的士宁的含量,为控制接骨丸的质量提供可靠地依据。方法利用高效液相色谱法,waters C18(250mm×4.6mm,5μm)反相色谱柱;流动相:乙腈:0.2%磷酸水溶液(20:70)等度洗脱;柱温:25℃;流速:1.0m L/min;检测波长:260nm。结果的士宁的线性范围是0.080mg/ml~0.400mg/ml;r值为0.99997,平均加样回收率为99.30%,RSD为0.89%。结论利用高效液相色谱法测定接骨丸中的士宁的含量,具有良好的重现性和专属性,且准确快捷,能够很好地控制接骨丸的内在质量。
OBJECTIVE To determine the content of strychnine contained in the main ingredient contained in Borneol by using high performance liquid chromatography (HPLC), and to provide a reliable basis for controlling the quality of Jie Gu Wan. Methods The mobile phase consisted of acetonitrile: 0.2% phosphoric acid (20:70) wasocratic elution was performed on a Waters C18 (250 mm × 4.6 mm, 5 μm) reversed- 1.0m L / min; detection wavelength: 260nm. The linear range was 0.080mg / ml ~ 0.400mg / ml. The r value was 0.99997. The average recovery was 99.30% and the RSD was 0.89%. Conclusions The determination of strychnine in Jiegu pill by high performance liquid chromatography has good reproducibility and specificity, accurate and fast, and can well control the intrinsic quality of Jiegu pill.