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本实验以异丙基肾上腺素心肌缺血(ISP—MI)为模型,对心肌膜酶、膜脂和自由基系统进行研究,探讨瓜蒌抗心肌缺血的机制。结果发现,ISP—MI后心肌游离脂肪酸(FFA)增加(P<0.05),心、肝、肾、脑和血清脂质过氧化产物丙二醛(MDA)含量增加(P<0.01)。心肌超氧化物岐化酶(SOD)和谷胱甘肽过氧化物酶(GSH—PX)活性均增高(P<0.05),Na~+—K~+ATP酶活性降低(P<0。05),Ca~(+++)-Mg~(++)ATP酶活性增高(P<0.05)。瓜蒌治疗后心肌MDA含量降低(P<0.01),Ca~(++)-Mg~(++)ATP酶活性增高(P<0.05),FFA含量减少(P<0.05)。表明瓜蒌对缺血心肌保护的机制与抗自由基损伤、减轻细胞内Ca~(++)超负荷及减少FFA有关。
In this study, isoproterenol-induced myocardial ischemia (ISP-MI) as a model of myocardial membrane enzymes, membrane lipid and free radical system to study the mechanism of melon stirrup against myocardial ischemia. The results showed that myocardial free fatty acid (FFA) increased after ISP-MI (P <0.05), and MDA content in heart, liver, kidney, brain and serum increased (P <0.01). Myocardial superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activity increased (P <0.05), Na ~ + -K ~ + ATPase activity decreased (P <0.05 ), Ca ~ (+++) - Mg ~ (++) ATPase activity increased (P <0.05). The content of MDA decreased (P <0.01), the activity of Ca ~ (++) - Mg ~ (++) ATPase increased (P <0.05) and the content of FFA decreased (P <0.05). The results showed that the protective mechanism of melon on ischemic myocardium was related to anti-free radical injury, alleviation of intracellular Ca ~ (++) overload and reduction of FFA.