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目的:探讨咳喘宁含药血清对呼吸道合胞病毒诱发大鼠哮喘模型CD4+T、CD8+T细胞内PI3K、STAT6的干预作用。方法:制作呼吸道合胞病毒诱发大鼠哮喘模型,提取并培养CD4+T、CD8+T细胞,采用RT-PCR和WesternBlot方法观察咳喘宁含药血清对呼吸道合胞病毒诱发大鼠哮喘模型CD4+T、CD8+T细胞内PI3K、STAT6的干预作用。结果:咳喘宁组PI3K m RNA、STAT6 m RNA表达量均较模型组和阳性药组降低,差异有统计学意义(P<0.05)。咳喘宁组PI3K蛋白、STAT6蛋白的相对表达量均较模型组和阳性药组降低,差异有统计学意义(P<0.05)。结论:咳喘宁含药血清对呼吸道合胞病毒诱发哮喘模型大鼠CD4+T、CD8+T细胞内PI3K、STAT6及其m RNA过高的表达产生明显抑制作用,从而纠正Th2细胞的过度增殖分化,影响Th2免疫应答优势,可能为其防治哮喘的作用机制之一。
Objective: To investigate the effect of Kechuanning medicated serum on respiratory syncytial virus-induced asthma in CD4 + T, CD8 + T cells PI3K, STAT6 intervention. Methods: The model of respiratory syncytial virus-induced asthma in rats was established. The CD4 + T and CD8 + T cells were extracted and cultured. The effect of Kechuanning containing serum on respiratory syncytial virus-induced asthma model CD4 + T, CD8 + T cells PI3K, STAT6 intervention. Results: The expression of PI3K m RNA and STAT6 m RNA in Kechuanning group were significantly lower than those in model group and positive control group (P <0.05). Kechuanning group PI3K protein, STAT6 protein relative expression levels than the model group and positive drug group decreased, the difference was statistically significant (P <0.05). Conclusion: Kechuanning containing serum can significantly inhibit the expression of PI3K, STAT6 and m RNA in CD4 + T and CD8 + T cells of asthmatic rats induced by respiratory syncytial virus, so as to correct the over proliferation of Th2 cells Differentiation, the impact of Th2 immune response advantages may be one of its mechanisms of prevention and treatment of asthma.