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采用卵黄囊组织切片、涂片的形态学、细胞化学染色、造血干/祖细胞体外培养及CD_(34)单克隆抗体免疫荧光检测等方法研究表明:人卵黄囊中存在造血岛,造血岛内由于造血微环境的特点致使此期造血主要向红系分化。血岛中检测出CD_(34)~+细胞,比例高于胎肝及成人骨髓,干/祖细胞于体外培养形成红系集落。结论:人胚胎期造血源于卵黄囊。
Yolk sac tissue sections, smear morphology, cytochemical staining, hematopoietic stem / progenitor cell culture in vitro and CD34 monoclonal antibody immunofluorescence assay showed that: human yolk sac has hematopoietic island, hematopoietic island Due to the characteristics of hematopoietic microenvironment caused by the current hematopoietic erythroid differentiation. CD34 (+) cells were detected in the blood island, the proportion was higher than fetal liver and adult bone marrow, and stem / progenitor cells were cultured in vitro to form erythroid colonies. Conclusion: Human embryonic hematopoiesis originated from yolk sac.