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建立人体细胞体外恶性转化系统尽管存在不少困难,但无论在阐明癌变机制和检测环境可疑致癌物方面都具有不可取代的价值。因此,我们自1975年起,在国内开创了人体细胞体外恶性转化和去恶化研究。(1)胎儿肺成纤维细胞体外用二乙基亚硝胺和一种新亚硝胺(甲基丁基甲基丙酮基亚硝胺)诱发恶性转化。亚硝胺为间接致癌物,能加大鼠肝P-450酶活化。在致癌物作用之前,先用软琼脂筛出克隆原干细胞加以处理,从而提高了转化机率。异种接种长出纤维肉瘤。(2)胎儿肾上皮细胞用二乙基亚硝胺诱发恶性转化获得成功。瓶中成片的培养
Although there are many difficulties in establishing a malignant transformation system of human cells in vitro, it has an irreplaceable value both in elucidating the mechanism of canceration and in detecting the suspected carcinogens in the environment. Therefore, since 1975, we have initiated research on malignant transformation and degeneration of human cells in vitro. (1) Fetal lung fibroblasts induce malignant transformation in vitro with diethylnitrosamine and a new nitrosamine (methylbutylmethyl acetonyl nitrosamine). Nitrosamines are indirect carcinogens that can be activated by rat liver P-450 enzymes. Before the action of the carcinogen, the cloned primitive stem cells were first screened out with soft agar to increase the conversion rate. Xenografts inoculated fibrosarcoma. (2) Fetal renal epithelial cells were successfully transformed with diethylnitrosamine induced malignant transformation. Bottles of cultured tablets