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目的研究海洋细菌Bacillus subtilis发酵液的化学成分。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及中低压制备柱色谱进行分离纯化,通过波谱方法进行结构鉴定。结果从发酵液中分离得到11个化合物,结构鉴定为3-苄基-6-异丙基哌嗪-2,5-二酮(Ⅰ)、3-异丁基-8-羟基吡咯并哌嗪-2,5-二酮(Ⅱ)、苯丙酸(Ⅲ)、3-异丁基吡咯并哌嗪-2,5-二酮(Ⅳ)、3-苄基吡咯并哌嗪-2,5-二酮(Ⅴ)、胸腺嘧啶(Ⅵ)、3-苄基哌嗪-2,5-二酮(Ⅶ)、尿嘧啶(Ⅷ)、二氢胸腺嘧啶(Ⅸ)、3-甲基-8-羟基吡咯并哌嗪-2,5-二酮(Ⅹ)、3-羟甲基吡咯并哌嗪-2,5-二酮(Ⅺ)。结论化合物Ⅰ,Ⅸ,Ⅹ和Ⅺ为首次从该属细菌中分离得到。
Objective To study the chemical composition of the fermentation broth of marine bacteria Bacillus subtilis. Methods Silica gel column chromatography, Sephadex LH-20 gel column chromatography and low pressure preparative column chromatography were used for separation and purification. Structure identification was performed by spectroscopic methods. Results Eleven compounds were isolated from the fermentation broth and identified as 3-benzyl-6-isopropylpiperazine-2,5-dione (I), 3-isobutyl-8-hydroxypyrrolo-piperazine. -2,5-dione (II), phenylpropionic acid (III), 3-isobutylpyrrolo-piperazine-2,5-dione (IV), 3-benzylpyrrolopyrazine-2,5 - Dione (V), Thymine (VI), 3-Benzylpiperazine-2,5-dione (VII), Uracil (VIII), Dihydrothymine (IX), 3-Methyl-8 -Hydroxypyrrolo-piperazin-2,5-dione (X), 3-Hydroxymethylpyrrolo-piperazine-2,5-dione (XI). Conclusion Compounds I, IX, X and XI were isolated from this genus for the first time.