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用遗传失活的团头鲂(Megalobrama amblycephala)的精子诱导红鲫(Carassius auratus Red Variety)进行雌核发育的研究,红鲫的卵子经适宜UV剂量灭活处理的团头鲂精子激活后,在0—4℃下冷休克40min抑制第二极体的排出使染色体加倍,获得雌核发育红鲫,它们的受精率、孵化率及成活率分别为(52．6±3．0)%,(23．6±4．1)%和(15．7±3．4)%,其成活率明显高于用鲤鱼精子诱导红鲫雌核发育的成活率(11．3±2．2)%．以外形特征、染色体数目及性腺发育程度为依据对雌核发育红鲫与对照杂交鱼进行了区分和鉴定,结果表明,Ⅰ龄鱼的体色为红色,染色体数目为100,性腺发育正常的个体为成功的雌核发育红鲫；而染色体数目为124或148,性腺发育滞后的个体为杂交鱼,其中三倍体鲫鲂是不育的(2年观察证明),而四倍体鲫鲂2年才能达到性成熟．以鲤鱼的精子作为对照,着重讨论了团头鲂精子作为刺激源的优势,即能提高雌核发育鱼的成活率,并可简化对雌核发育鱼的鉴定,研究证明团头鲂的精子是一种非常有效的诱导鲫鱼进行雌核发育的刺激源。
Gynogenetic studies using the genetically inactivated Megalobrama amblycephala sperm induced by Carassius auratus Red Variety showed that when the eggs of red crucian carp were activated by appropriate UV dose inactivation, Cold shock at 0-4 ℃ for 40 min inhibited the excretion of the second polar body and doubled chromosomes to obtain gynogenetic red crucian carp. The fertilization rate, hatching rate and survival rate were (52.6 ± 3.0)%, ( 23.6 ± 4.1)% and (15.7 ± 3.4)%, respectively. The survival rate was significantly higher than that of carp spermatozoa (11.3 ± 2.2)%. Based on the morphological features, chromosome number and gonadal development, the differentiation and identification of gynogenetic red crucian carp and control crossbred fish were studied. The results showed that the body color of the first instar fish was red, the number of chromosomes was 100, and the individuals with normal gonadal development The results showed that for the successful gynogenetic red crucian carp (Carassius auratus gigas), the number of chromosomes was 124 or 148, while those with delayed gonadal development were hybrid fish. The triploid Carassius auratus was sterile (2 years of observation), while the tetraploid Carassius auratus 2 Years to achieve sexual maturity. Carp sperm as a control, focusing on discussing the advantages of bream sperm as a stimulus, which can improve the survival rate of gynogenetic fish, and can simplify the identification of gynogenetic fish, studies have shown that the sperm of the bream is A very effective stimulus to induce gynogenetic gynogenesis.