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目的研究熊果酸(Ursolic Acid,UA)的致突变作用及其拮抗作用。方法采用小鼠骨髓嗜多染红细胞微核实验、小鼠染色体畸变实验、小鼠精子畸形实验和改进的小鼠骨髓嗜多染红细胞微核实验、染色体畸变实验、小鼠精子畸形实验,通过检测骨髓嗜多染红细胞微核率、染色体畸变率和精子畸形率来研究熊果酸的致突变性和抗突变性。结果熊果酸各致突变实验组(HUA、MUA、LUA)与阳性对照组(环磷酰胺,CP)相比,差异有统计学意义(P<0.05),表明熊果酸没有致突变性;熊果酸抗突变实验组(HUA+CP、MUA+CP和LUA+CP)与阳、阴性对照组相比,差异有统计学意义(P<0.05),表明熊果酸可降低环磷酰胺诱发的细胞微核、染色体畸变率和精子畸形率。结论在本试验条件下,熊果酸无致突变作用,具有一定的拮抗作用。
Objective To study the mutagenicity and antagonism of ursolic acid (UA). Methods The mouse bone marrow polychromatic erythrocyte micronucleus test, mouse chromosome aberration test, mouse sperm abnormality test and modified mouse bone marrow polychromatic erythrocyte micronucleus test, chromosome aberration test and mouse sperm abnormality test were adopted. Bone marrow micronucleus rate of polychromatic erythrocytes, chromosome aberration rate and sperm deformity rate to study the ursolic acid mutagenicity and anti-mutation. Results There was a significant difference (P <0.05) between ursolic acid mutagenicity test group (HUA, MUA, LUA) and positive control group (cyclophosphamide, CP) Compared with the positive control and negative control, HUA + CP, MUA + CP and LUA + CP showed significant difference (P <0.05), indicating that ursolic acid can reduce the induction of cyclophosphamide Micronuclei, chromosome aberration rate and sperm deformity rate. Conclusion Under the test conditions, ursolic acid has no mutagenic effect and has some antagonistic effects.