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本文以光敏感核不育水稻农垦58-s 和常规水稻农垦58为材料,从第一次枝梗原基分化后期至花粉母细胞形成期用不同光周期(SD 和 LD)进行育性诱导处理,半微量的二硝基水扬酸法定糖结果表明:1.从第一次枝梗原基分化至雌雄蕊形成期,农垦58(SD~(**)或 LD~(***))的淀粉含量逐渐增加,蔗糖和还原糖含量经过一个相对稳定的阶段(Ⅱ—Ⅲ)后,迅速增加,至雌雄蕊形成期时,淀粉、蔗糖和还原糖含量达到高峰,随后逐渐下降。2.从第一次枝梗原基分化至雌雄蕊形成期,农垦58-s(SD 或 LD)的淀粉、蔗糖和还原糖含量的变化规律与农垦58一致。但是,在雌雄蕊形成期之后,农垦58-sSD~(****)的蔗糖含量下降,淀粉和还原糖含量缓慢增加,而农垦58-sLD~(*****)的淀粉、蔗糖和还原糖含量均明显增加。推测长日照可能通过某些代谢环节影响了农垦58-s 叶片内碳水化合物向雄蕊的运输。营养缺乏是造成小饱子败育的重要原因之一。
In this study, the photoperiod-sensitive male sterile Nongken 58-s and conventional rice Nongken 58 were used as fertilizers to induce fertility in different photoperiod (SD and LD) from the initial stage of primordial differentiation of stems to the formation of pollen mother cells , Semi-trace dinitro-salicylic acid statutory sugar results show that: 1. From the first branch primordia to the formation of stamens, Nongken 58 (SD ~ (**) or LD ~ (***)) The content of starch, sucrose and reducing sugar reached the peak at the formation stage of the female and the stamen, then gradually decreased. The content of sucrose and reducing sugar increased rapidly after a relatively stable stage (Ⅱ-Ⅲ). The change of starch, sucrose and reducing sugar content of Nongken 58-s (SD or LD) was consistent with that of Nongken 58 from the first branch primordium differentiation to the formation of female and stamen. However, the sucrose content of Nongken 58-sSD ~ (****) decreased and the content of starch and reducing sugar slowly increased after the formation of pistils and stamens, while the Nongken 58-sLD ~ (*****) starch, sucrose And reducing sugar content increased significantly. Speculated that the long sunshine may affect some aspects of the metabolism of Nongken 58-s leaf carbohydrate transport to the stamens. Nutritional deficiencies are one of the important reasons for the abortion of the small ones.