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目的探讨铅暴露对小鼠胸腺细胞胸腺内迁移相关趋化性细胞因子受体的影响。方法将48只健康初断乳21日龄清洁级SD小鼠随机分为4组,分别为对照(蒸馏水)组和低(200 mg/L)、中(400 mg/L)、高(800 mg/L)剂量乙酸铅染毒组,每组12只,雌雄各半。采用自由饮水方式进行染毒,连续染毒12周。染毒结束后,采用石墨炉原子吸收法检测血清、胸腺中的铅含量,采用流式细胞技术检测小鼠胸腺细胞趋化因子受体CXCR4、CCR5、CCR9的表达。结果与对照组比较,各剂量乙酸铅染毒组小鼠血清、胸腺铅含量以及小鼠胸腺细胞膜分子CXCR4、CCR5、CCR9的表达均明显升高,差异有统计学意义(P<0.05);且随着乙酸铅染毒剂量的升高,小鼠血清、胸腺铅含量以及小鼠胸腺细胞膜分子CXCR4、CCR5、CCR9的表达呈上升趋势。结论趋化性细胞因子受体参与长期铅暴露小鼠胸腺的免疫损伤。
Objective To investigate the effect of lead exposure on chemokine receptor related to thymus migration in mouse thymus. Methods 48 SD healthy mice were randomly divided into 4 groups: control (distilled water), low (200 mg / L), medium (400 mg / L) and high / L) dose of lead acetate exposure group, each group of 12, male and female. Adopt free drinking water to carry on poisoning, continuous poisoning for 12 weeks. At the end of exposure, the content of lead in serum and thymus was detected by graphite furnace atomic absorption spectrometry. The expression of chemokine receptors CXCR4, CCR5 and CCR9 in mouse thymocytes was detected by flow cytometry. Results Compared with the control group, the contents of lead and serum thymus and the expression of CXCR4, CCR5 and CCR9 in thymus membrane of mice induced by lead acetate were significantly increased (P <0.05); and With the increase of the dose of lead acetate, the serum levels of thymus and the contents of CXCR4, CCR5 and CCR9 in mouse thymus cells increased. Conclusions Chemotactic cytokine receptors are involved in the immunostaining of the thymus in mice exposed to chronic lead exposure.