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X线显微射线摄影术的标本制作和一般光学显微镜的有所不同,它不是观察一般组织学结构,而是研究所含矿物性成分,如钙的分布和含量。标本的制作要求很高,骨或牙不经脱钙磨成40—100微米的薄片,厚度必须一致,其误差不得超过5％微米,因标本是直接用X射线照射,照射后的底片经显像及定影。用显微镜观察底片上的物相各部位的黑度,同时用显微光度计测量其黑度的深浅,这样就能作钙的定量。为了精确测量矿物质含量,故整个标本的厚度必须一致,同时还要求个个标本厚度相同,而且两面必须光滑无磨琢痕迹及裂隙才符合标准。要达到上述要求,按一般骨磨片方法是不能满足
X-ray photomicrography specimen preparation and general optical microscope is different, it is not to observe the general histological structure, but to study the mineral components contained, such as the distribution and content of calcium. Specimens are highly demanded for production. Bone or teeth are decalcified to a thickness of 40-100 microns without decay. The thickness must be the same, with an error of no more than 5%. The specimen is irradiated directly with X-rays. Like and fixing. With a microscope to observe the blackness of the various parts of the film on the film, while using a photometer to measure the depth of its darkness, so that you can make calcium quantitative. In order to accurately measure the mineral content, the thickness of the entire specimen must be the same, but also requires the same thickness of all specimens, and both sides must be smooth without grinding marks and cracks to meet the standards. To achieve the above requirements, according to the general method of bone grinding is not satisfied