论文部分内容阅读
将体质量100~120 g的雄性SD大鼠258只随机分为7组。Ⅰ组,0.3 mg/kg富硒麦芽硒组;Ⅱ组,1 mg/kg富硒麦芽硒组;Ⅲ组,3 mg/kg富硒麦芽硒组;Ⅳ组,3 mg/kg亚硒酸钠硒组;Ⅴ组,诱癌阳性对照组;Ⅵ组,3 mg/kg亚硒酸钠硒不诱癌组;Ⅶ组,阴性对照组,不加硒也不诱癌。Ⅰ~Ⅴ组,饮水中添加二乙基亚硝胺(DENA,100 mg/L)以诱癌。每2周眼眶采血1次,测定血浆丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、总胆红素(TBIL)、白蛋白(ALB)、葡萄糖和血清钙含量、全血GSH-Px活性等。结果发现,富硒麦芽硒比亚硒酸钠硒能更有效地抑制DENA所致大鼠肝损伤,提高大鼠全血GSH-Px活性,并以1 mg/kg添加剂量效果较佳;DENA所致大鼠肝肿瘤初期即伴随着明显的低血糖症。结果表明:富硒麦芽比亚硒酸钠能更有效抑制DENA所致的初期肝损伤,保护肝脏,提高抗氧化能力。
258 male SD rats weighing 100-120 g were randomly divided into 7 groups. Group Ⅰ, 0.3 mg / kg selenium-enriched maltose group; Group Ⅱ, 1 mg / kg selenium-enriched maltose group; Group Ⅲ, 3 mg / kg selenium-enriched maltose group; Selenium group, Ⅴ group, positive control group; Ⅵ group, 3 mg / kg sodium selenite selenium did not induce cancer group; Ⅶ group, negative control group, without selenium or cancer. Groups Ⅰ to Ⅴ, drinking water added diethylnitrosamine (DENA, 100 mg / L) to induce cancer. Blood samples were taken from the orbital cavity every 2 weeks to measure plasma ALT, ALP, TBIL, ALB, glucose and serum calcium, whole blood GSH-Px activity and so on. The results showed that selenium-enriched selenium selenite selenium can more effectively inhibit DENA-induced liver injury in rats and improve GSH-Px activity in rat whole blood, and the dose of 1 mg / kg is better; DENA The initial liver tumor in rats is accompanied by obvious hypoglycemia. The results showed that selenium-enriched malt than sodium selenite can effectively inhibit the initial liver injury caused by DENA, protect the liver and improve the anti-oxidative capacity.