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本研究用构成核酸的嘌呤核苷酸代谢为模型了解胎儿代谢的机理,合成系统以脱氢酶(IMPD),分解系统以黄嘌呤氧化酶(XO)为代表.实验采用Wister HLA系大鼠,饲养于室温25±1℃,湿度60±10%,12小时明暗交替,固体形饲料,自由摄水.11周龄开始交配,出现阴道体栓时为妊娠0日.取胎仔时用戊巴比妥钠腹腔内注射麻醉母鼠;剖腹取出.取肝组织时,先断头泻血后再取.为防止时间差异,操作均在上年10～11时进行.
In this study, purine nucleotides metabolism of nucleic acid was used as a model to understand the mechanism of fetal metabolism, and the synthesis system was represented by IMPD and xanthine oxidase (XO) .Wister HLA rats, Reared in room temperature 25 ± 1 ℃, humidity 60 ± 10%, 12 hours of light and dark alternating solid feed, free water intake .11 weeks of age began to mate, when the vaginal suppository for pregnancy 0. Take fetus Aberdeen with pentobarbital Intraperitoneal sodium anesthesia anesthesia female rats; Caesarean section to take out. Take liver tissue, the first off the head and spilled blood and then take.In order to prevent the time difference, the operation was carried out 10 to 11 in the previous year.