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目的:观察贝那普利及维生素E对糖尿病肾病(DN)大鼠肾脏一氧化氮合成酶(NOS)活性、一氧化氮(NO),超氧化物歧化酶(SOD)、丙二醛(MDA)含量及血清总抗氧化能力的影响,探讨两者在DN中的可能作用机制。方法:注射链佐脲菌素制作DN模型,分为4组,A组:正常对照组;B组:糖尿病肾痛模型组;C组:贝那普利治疗组;D组:贝那普利+维生素E治疗组。于第12周检测24h尿蛋白定量(TP)、血清胱蛋白酶抑制剂C(Cys C)、糖化血红蛋白(HbAlc),血清总抗氧化能力,肾组织NOS、NO、SOD、MDA含量。结果:与A组相比,B组TP、CysC、HbAlc、肾组织血糖、MDA明显升高(P<0.01),而血清总抗氧化能力,肾组织NO、NOS、SOD明显降低(P<0.01)。与B组相比,C组TP、CysC、HbAlc、肾组织血糖显著降低(P<0.01),MDA亦下降(P<0.05),而总抗氧化能力增强(P<0.05),SOD升高(P<0.01)。与C组相比,D组TP、肾组织血糖下降更加显著(P<0.01),MDA下降(P<0.05),而NO、NOS、SOD明显升高(P<0.05或P<0.01),总抗氧化能力增强(P<0.05)。结论:贝那普利能降低TP、HbAlc、肾组织血糖、MDA,增加NO、NOS、SOD,维生素E可增加血清总抗氧化能力,清除自由基而有肾保护作用。两者对治疗DN氧化应激有协同作用。
AIM: To observe the effects of benazepril and vitamin E on the activity of nitric oxide synthase (NOS), nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA) in diabetic nephropathy rats ) Content and total serum antioxidant capacity, to explore the possible mechanism of the two in DN. Methods: DN model was made by injecting streptozotocin in streptozotocin (DN) model and divided into 4 groups: group A: normal control group; group B: diabetic nephropathy model group; group C: benazepril treatment group; group D: benazepril + Vitamin E treatment group. At the 12th week, 24 h urinary protein (TP), Cys C, HbAlc, total serum antioxidant capacity, NOS, NO, SOD and MDA contents in renal tissue were measured. Results: Compared with group A, serum TP, CysC, HbAlc, blood glucose and MDA in group B were significantly increased (P <0.01), but serum total antioxidant capacity and NO, NOS and SOD in renal tissue were significantly decreased ). Compared with group B, the levels of TP, CysC, HbAlc and renal tissue blood glucose in group C were significantly decreased (P <0.01), MDA also decreased (P <0.05), total antioxidant capacity P <0.01). Compared with group C, the blood glucose of TP and renal tissue in group D decreased more significantly (P <0.01) and MDA decreased (P <0.05), while the levels of NO, NOS and SOD were significantly increased in group D (P <0.05 or P <0.01) Antioxidant capacity increased (P <0.05). Conclusion: Benazepril can reduce TP, HbAlc, renal blood glucose, MDA, increase NO, NOS, SOD, vitamin E can increase serum total antioxidant capacity, scavenging free radicals and kidney protection. Both have a synergistic effect on the treatment of DN oxidative stress.