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目的:观察异氟醚和地尔硫卓联合应用在体外对人精子运动功能的影响并探讨其机制。方法:选取10例活力正常的精液标本,每例精液均一式9份,1份作为对照,其余8份分别经过不同浓度地尔硫卓单独或联合应用4.2%异氟醚处理1 h,采用计算机辅助精液分析系统观察精子运动功能的变化情况。结果:①与对照组相比,0.01~1 g/L地尔硫卓均可显著降低精子活力,并呈浓度依赖性降低,当地尔硫卓浓度升高至1 g/L,精子活力显著降低。②将不同浓度的地尔硫卓和4.2%异氟醚联合应用后与同浓度下地尔硫卓组对比,0.01 g/L地尔硫卓与异氟醚联合用药时精子的活力显著升高,0.04 g/L地尔硫卓与异氟醚联合用药时精子的活力无显著差别,0.2 g/L地尔硫卓与异氟醚联合用药时精子的活力显著降低,1 g/L地尔硫卓联合用药时精子的活力无显著差别。结论:异氟醚兴奋精子运动的作用可能与精子的钙离子通道有关,当此兴奋作用被地尔硫卓完全阻断时,异氟醚对精子的运动则表现出抑制作用。
Objective: To observe the effect of isoflurane combined with diltiazem on the motility of human sperm in vitro and its mechanism. Methods: Semen samples of 10 cases with normal vitality were selected. Each sample of semen was homogenized with 9 samples and 1 sample as control. The remaining 8 samples were treated with different concentrations of diltiazem alone or in combination with 4.2% isoflurane for 1 h. The computer-assisted semen analysis Systematic observation of changes in sperm motility function. RESULTS: Compared with the control group, diltiazem 0.01-1 g / L significantly reduced the sperm motility and decreased the concentration of stilbazol in a dose-dependent manner. When the concentration of diltiazem was increased to 1 g / L, sperm motility was significantly decreased. ② The combination of diltiazem with different concentrations and 4.2% isoflurane compared with diltiazem group at the same concentration, 0.01g / L diltiazem combination with isoflurane significantly increased sperm motility, 0.04g / L diltiazem and isoflurane There was no significant difference in sperm motility when combined with ether. The sperm motility was significantly decreased when 0.2 g / L diltiazem was combined with isoflurane. There was no significant difference in sperm motility when combined with 1 g / L diltiazem. CONCLUSION: The effect of isoflurane on sperm motility may be related to sperm calcium channel. When isoflurane completely blocked by diltiazem, isoflurane exerts inhibitory effect on sperm motility.